The singer announced the money would be given to the LA Fire Relief Fund through her BeyGOOD Foundation to help victims of the wildfires that have devastated large swathes of southern California.
"Los Angeles we stand with you," a message posted on the charity's Instagram page said.
"BeyGOOD is taking action by announcing the LA Fire Relief Fund with a donation of $US2.5 million ($A4.1 million).
"The fund is earmarked to aid families in the Altadena/Pasadena area who lost their homes, and to churches and community centres to address the immediate needs of those affected by the wildfires."
Beyonce's donation comes after the Walt Disney Company pledged $US15 million ($A24 million) to help rebuild communities devastated by the fires while Paramount and Fox Corp. handed over $US1 million ($A1.6 million) in contributions to the American Red Cross and the Los Angeles Fire Department.
The fires broke out on Tuesday and at least 24 people have been confirmed dead with thousands more losing their homes - including celebrities Mel Gibson, Paris Hilton and Billy Crystal.
Meghan, Duchess of Sussex and her husband Prince Harry, Duke of Sussex have been among the famous faces trying to help out with the royals joining efforts to distribute food and supplies to victims of the wildfires in Pasadena, where they also thanked first responders and met with affected families.
The couple live in Montecito - north of the affected area - and are believed to have offered their $US14 million ($A23 million) mansion as a refuge to friends fleeing the fires, which have left more than 100,000 people displaced.
The duchess has also announced she's delaying the launch of her new Netflix series With Love, Meghan because of the disaster.
The show had been due to hit screens on Wednesday but at her request, the streaming service will now hold back the lifestyle show until March 4.
"I'm thankful to my partners at Netflix for supporting me in delaying the launch, as we focus on the needs of those impacted by the wildfires in my home state of California," she said in a statement."